Journal: Oncotarget
Article Title: Overexpression of HE4 (human epididymis protein 4) enhances proliferation, invasion and metastasis of ovarian cancer
doi:
Figure Lengend Snippet: Quantitative real-time PCR for selected genes (IL1A, DUSP1, COL1A1 and ITGB5) found to be differentially expressed in gene microarrays. Both in ES-2 cells and Caov-3 cells, the relative mRNA expression of 4 genes was significantly higher in HE4 high expressed cells and lower in HE4 low expressed cells (panel A, compared with Mock cells, one-way ANOVA). ICC staining showed that integrin β5 was highly expressed in HE4-high expression cells, relatively low expressed in the mock cells (HE4-H-Mock and HE4-L-Mock) and sparsely expressed in HE4 low expression cells (panel B) Immunohistochemical staining in xenograft tumor tissues showed that integrin β5 was high expressed in all the HE4 highly expressed tumor tissues, relatively low expressed in the HE4-H-Mock tumors and totally not expressed in HE4-L tumors (100%, 80% and 0% in 3 groups, respectively, P=0.003, panel C). IHC staining of ovarian cancer sample showed that expressions of HE4 and integrin β5 were mainly on membrane and cytoplasm, their expressions were relatively lower in chemotherapy sensitive group than the resistant group, the correlation coefficient was 0.213 (Spearman correlation analysis, P = 0.042, panel D). Kaplan-Meier survival analysis (panel E) showed that high expressions of HE4 and integrin β5 were independent risk factors for overall survival (OS) and progression-free survival (PFS), log Rank P = 0.001, 0.001 for HE4 in OS and PFS, P = 0.029, 0.042 for integrin β5 in OS and PFS, respectively.
Article Snippet: Rabbit polyclonal anti-HE4 antibody and rabbit polyclonal anti-integrin β5 antibody were purchased from Abcam Company (USA).
Techniques: Real-time Polymerase Chain Reaction, Expressing, Staining, Immunohistochemical staining, Immunohistochemistry, Membrane